Publications

2010

Baumann M, Pontiller J, Ernst W. (2010) Structure and basal transcription complex of RNA polymerase II core promoters in the mammalian genome: an overview. Mol Biotechnol, 45(3):241-7.
Pontiller J, Maccani A, Baumann M, Klancnik I, Ernst W. (2010) Identification of CHO endogenous gene regulatory elements. Mol Biotechnol, 45(3):235-40.
Krammer F, Schinko T, Palmberger D, Tauer C, Messner P, Grabherr R. (2010) Trichoplusia ni cells (High Five) are highly efficient for the production of influenza A virus-like particles: a comparison of two insect cell lines as production platforms for influenza vaccines. Mol Biotechnol, 45(3):226-34.
Krammer F, Schinko T, Messner P, Palmberger D, Ferko B, Grabherr R. (2010) Influenza virus-like particles as an antigen-carrier platform for the ESAT-6 epitope of Mycobacterium tuberculosis. J Virol Methods, 167(1):17-22.
Grabherr R, Ernst W. (2010) Baculovirus for eukaryotic protein display. Curr Gene Ther. ,10(3):195-200.
Mairhofer J., Cserjan-Puschmann M., Striedner G., Nöbauer K., Razzazi-Fazeli E., Grabherr R. (2010) Marker-free plasmids for gene therapeutic applications - lack of antibiotic resistance gene substantially improves the manufacturing process. J Biotechnol, 146(3):130-7.
Krammer F., Nakowitsch S., Messner P., Palmberger D., Ferko B., Reingard R. (2010) Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice. Biotechnol J, 5, 17-23.

2009

Heinl S., Hartinger D., Thamhesl M., Vekiru E., Krska R., Schatzmayr G., Moll W.D., Grabherr, R (2009) Degradation of fumonisin B(1) by the consecutive action of two bacterial enzymes. J Biotechnol, 2, 120-129
Mairhofer J., Roppert K., Ertl P. (2009) Microfluidic Systems for Pathogen Sensing: A Review. Sensors, 9(6), 4804-4823.

2008

Pontiller, J., Gross, S., Thaisuchat, H., Hesse, F. and Ernst, W. (2008) Identification of CHO Endogenous Promoter Elements Based on a Genomic Library Approach. Mol Biotechnol.
Mairhofer, J. and Grabherr, R. (2008) Rational Vector Design for Efficient Non-viral Gene Delivery: Challenges Facing the Use of Plasmid DNA. Mol Biotechnol.
Mairhofer, J., Pfaffenzeller, I., Merz, D. and Grabherr, R. (2008) A novel antibiotic free plasmid selection system: advances in safe and efficient DNA therapy. Biotechnol J, 3, 83-89.

2006

Fischlechner, M., Toellner, L., Messner, P., Grabherr, R. and Donath, E. (2006) Virus-engineered colloidal particles--a surface display system. Angew Chem Int Ed Engl, 45, 784-789.
Matilainen, H., Mäkelä, A., Riikonen, R., Saloniemi, T., Korhonen, E., Hyypiä, T., Heino, J., Grabherr, R. and Oker-Blom, C. (2006) RGD motifs on the surface of baculovirus enhance transduction of human lung carcinoma cells. J Biotechnol, 125, 114-126.
Ernst, W., Schinko, T., Spenger, A., Oker-Blom, C. and Grabherr, R. (2006) Improving baculovirus transduction of mammalian cells by surface display of a RGD-motif. J Biotechnol, 126, 237-240.
Toellner, L., Fischlechner, M., Ferko, B., Grabherr, R. and Donath, E. (2006) Virus-coated layer-by-layer colloids as a multiplex suspension array for the detection and quantification of virus-specific antibodies. Clin Chem, 52, 1575-1583.
Pfaffenzeller, I., Mairhofer, J., Striedner, G., Bayer, K. and Grabherr, R. (2006) Using ColE1-derived RNA I for suppression of a bacterially encoded gene: implication for a novel plasmid addiction system. Biotechnol J, 1, 675-681.
Nakowitsch, S., Kittel, C., Ernst, W., Egorov, A. and Grabherr, R. (2006) Optimization of baculovirus transduction on FreeStyle293 cells for the generation of influenza B/Lee/40. Mol Biotechnol, 34, 157-164.

2004

Ojala, K., Koski, J., Ernst, W., Grabherr, R., Jones, I. and Oker-Blom, C. (2004) Improved display of synthetic IgG-binding domains on the baculovirus surface. Technol Cancer Res Treat, 3, 77-84.
Spenger, A., Ernst, W., Condreay, J., Kost, T. and Grabherr, R. (2004) Influence of promoter choice and trichostatin A treatment on expression of baculovirus delivered genes in mammalian cells. Protein Expr Purif, 38, 17-23.

2003

Poomputsa, K., Kittel, C., Egorov, A., Ernst, W. and Grabherr, R. (2003) Generation of recombinant influenza virus using baculovirus delivery vector. J Virol Methods, 110, 111-114.
Oker-Blom, C., Airenne, K. and Grabherr, R. (2003) Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery. Brief Funct Genomic Proteomic, 2, 244-253.

2002

Grabherr, R., Nilsson, E., Striedner, G. and Bayer, K. (2002) Stabilizing plasmid copy number to improve recombinant protein production. Biotechnol Bioeng, 77, 142-147.
Grabherr, R. and Bayer, K. (2002) Impact of targeted vector design on Co/E1 plasmid replication. Trends Biotechnol, 20, 257-260.
Spenger, A., Grabherr, R., Töllner, L., Katinger, H. and Ernst, W. (2002) Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64. Eur J Biochem, 269, 4458-4467.
Marzban, G., Grillari, J., Reisinger, E., Hemetsberger, T., Grabherr, R. and Katinger, H. (2002) Age-related alterations in the protein expression profile of C57BL/6J mouse pituitaries. Exp Gerontol, 37, 1451-1460.

2001

Grillari, J., Fortschegger, K., Grabherr, R., Hohenwarter, O., Kunert, R. and Katinger, H. (2001) Analysis of alterations in gene expression after amplification of recombinant genes in CHO cells. J Biotechnol, 87, 59-65.
Grabherr, R. and Ernst, W. (2001) The baculovirus expression system as a tool for generating diversity by viral surface display. Comb Chem High Throughput Screen, 4, 185-192.
Grabherr, R., Ernst, W., Oker-Blom, C. and Jones, I. (2001) Developments in the use of baculoviruses for the surface display of complex eukaryotic proteins. Trends Biotechnol, 19, 231-236.

2000

Grillari, J., Hohenwarter, O., Grabherr, R. and Katinger, H. (2000) Subtractive hybridization of mRNA from early passage and senescent endothelial cells. Exp Gerontol, 35, 187-197.
Ernst, W., Spenger, A., Toellner, L., Katinger, H. and Grabherr, R. (2000) Expanding baculovirus surface display. Modification of the native coat protein gp64 of Autographa californica NPV. Eur J Biochem, 267, 4033-4039.

1998

Ernst, W., Grabherr, R., Wegner, D., Borth, N., Grassauer, A. and Katinger, H. (1998) Baculovirus surface display: construction and screening of a eukaryotic epitope library. Nucleic Acids Res, 26, 1718-1723.

1997

Grabherr, R., Ernst, W., Doblhoff-Dier, O., Sara, M. and Katinger, H. (1997) Expression of foreign proteins on the surface of Autographa californica nuclear polyhedrosis virus. Biotechniques, 22, 730-735.

1996

Knezević, M., Vorauer-Uhl, K., Hohenwarter, O., Steindl, F., Grabherr, R. and Raspor, P. (1996) Human interleukin-2 (IL-2) expressed by transfected mammalian cells. Pflugers Arch, 431, R227-228.

1994

Ernst, W., Grabherr, R. and Katinger, H. (1994) Direct cloning into the Autographa californica nuclear polyhedrosis virus for generation of recombinant baculoviruses. Nucleic Acids Res, 22, 2855-2856.
Engelhardt, O., Grabherr, R., Himmler, G. and Rüker, F. (1994) Two-step cloning of antibody variable domains in a phage display vector. Biotechniques, 17, 44, 46.
Zhang, Y., Strasser, P., Grabherr, R. and Van Etten, J. (1994) Hairpin loop structure at the termini of the chlorella virus PBCV-1 genome. Virology, 202, 1079-1082.

1993

Nelson, M., Zhang, Y., Steffens, D., Grabherr, R. and Van Etten, J. (1993) Sequencing two DNA templates in five channels by digital compression. Proc Natl Acad Sci U S A, 90, 1647-1651.

1992

Nelson, M., Van Etten, J. and Grabherr, R. (1992) DNA sequencing of four bases using three lanes. Nucleic Acids Res, 20, 1345-1348.
Grabherr, R., Strasser, P. and Van Etten, J. (1992) The DNA polymerase gene from chlorella viruses PBCV-1 and NY-2A contains an intron with nuclear splicing sequences. Virology, 188, 721-731.

1990

Shields, S., Burbank, D., Grabherr, R. and van Etten, J. (1990) Cloning and sequencing the cytosine methyltransferase gene M. CviJI from Chlorella virus IL-3A. Virology, 176, 16-24.