Juergen Mairhofer

Education

• 2010  – present: Post Doctoral Research fellow at the Austrian Centre of Industrial Biotechnology (ACIB)
• 2007 – 2010: PhD thesis "Innovations in Plasmid Production and Gene Therapy - Improved Safety, Productivity and Efficiency"
• 2005 – 2006: Diploma thesis at the Baculo Virus Group of Prof. Reingard Grabherr, Institute of Applied Microbiology (IAM): “Improved antisense-constructs for targeted gene-silencing mediated by ColE1-type plasmid attendance - Towards the development of a novel antibiotic free plasmid selection system“
• 1999 – 2006: University of Natural Resources and Applied Life Sciences Vienna:  Food- and Biotechnology
• 1991 – 1999: Bundesrealgymnasium Rohrbach

Employment History

• August – October 2004:  Vogelbusch GmbH.,Vienna
• August – September 2003:  Kwizda Pharma, Linz
• July – August 2001:  Stiftsbrauerei Schlägl,  Schlägl

Publications

• Mairhofer J., Cserjan-Puschmann M., Striedner G., Nöbauer K., Razzazi-Fazeli E., Grabherr R.: Marker-free plasmids for gene therapeutic applications-Lack of antibiotic resistance gene substantially improves the manufacturing process. J Biotechnol 2010.
• Mairhofer J., Roppert K., Ertl P.: Microfluidic Systems for Pathogen Sensing: A Review. Sensors 2009, 9, 4804-4823.
• Mairhofer J., Grabherr R.: Rational vector design for efficient non-viral gene delivery: Challenges facing the use of plasmid DNA. Mol Biotech 2008, 39(2):97-104.
• Mairhofer J., Pfaffenzeller I., Merz D., Grabherr R.: A novel antibiotic free plasmid selection system: Advances in safe and efficient DNA therapy. Biotechnol J  2008,3:83-89.
• Pfaffenzeller I., Mairhofer J., Striedner G., Bayer K., Grabherr R.: Using ColE1-derived RNAI for supression of a bacterially encoded gene: Implication for a novel plasmid addiction system. Biotechnol J 2006, 1:675-681.

 Oral Presentations

• Mairhofer J., Pfaffenzeller I., Merz D., Huber H., Grabherr R.: Antibiotic-free Selection System for the Manufacturing of Minimized Plasmids. 2nd International Workshop on Minicircle DNA, Bielefeld, Germany, May 2008.

Training

• International Summer School on Advanced Techniques in Bacterial Genome Research,  28 Sept-3 Oct 2009, CeBiTec, University of Bielefeld.     
• Systems Biology Short Course, June 8-12 2009, Center for Systems Biology, University of Iceland, Reykjavik.

Posters

• Mairhofer J., Strobl F., Striedner G., Grabherr R.: Improving DNA-based therapies: design of novel host, vectors and production strategies for the manufacturing of antibiotic marker-free plasmids. 14th European Congress on Biotechnology, Barcelona, Spain ,Sep. 2009.
• Mairhofer J., Egger E., Pfaffenzeller I., Striedner G., Grabherr R.: Marker-Free Plasmid DNA Production - Advances in Safe and Efficient DNA Therapy. 5th Conference on Recombinant Protein Production (RPP), Alghero, Italy, Sep. 2008.
• Mairhofer J., Pfaffenzeller I., Merz D., Grabherr R.: Minimal bacterial plasmids with improved transformation efficiencies. 8th Conference on Protein Expression in Animal Cells (PEACe), Angra Dos Reis, Brasil, Sep. 2007.
• Keller D., Huber H., Mairhofer J., Pfaffenzeller I., Grabherr R.:  Development and Production of Marker-Free Non-Viral Vectors. 10th Annual Meeting of the American Society of Gene Therapy (ASGT), Seattle, USA, May 2007.
• Mairhofer J., Pfaffenzeller I., Merz D., Grabherr R.: A novel platform for the antibiotic-free maintenance of therapeutic plasmids. 3rd International Conferance on DNA Vaccines, Malaga, Spain, May 2007.
• Pfaffenzeller I., Mairhofer J., Striedner G., Bayer K., Grabherr R.: A novel approach for antibiotic free selection of recombinant plasmids for gene therapeutic applications based on RNA antisense reaction. Joint Annual Meeting of the ÖGBT, ÖGBM, ÖGGGT and ANGT, Salzburg, Austria, Sep. 2006.
• Pfaffenzeller I., Mairhofer J., Striedner G., Bayer K., Grabherr R.: A novel approach for antibiotic-free selection of recombinant plasmids for gene therapeutic applications based on RNA antisense reaction. 7th Conference on Protein Expression in Animal Cells (PEACe), Hersonnisos, Crete, Greece. Sep 2005.
• Mairhofer J., Pfaffenzeller I., Striedner G., Bayer K., Grabherr R.: Improved Antisense constructs for targeted gene-silencing mediated by ColE1-type plasmid attendance.    Joint Annual Meeting of the ÖGBT, ÖGBM, ÖGGGT and ANGT, Vienna. Sep. 2005.